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7 votes

Can a RT-PCR test claim 100% sensitivity and specificity?

A diagnostic test should never claim 100% sensitivity or specificity. So we are all on the same page, let's review what sensitivity and specificity actually mean. According to the US Food and Drug ...
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4 votes

Why are COVID nucleic acid tests sometimes reported with non-integer CT values?

Viral nucleic acid amplification tests use real time PCR to detect viral genomic material. If the genetic material is present, a reaction will occur that generates fluorescence. If sample produces a ...
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2 votes
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How long it will take for a RT-PCR test CT value to go to from 27 to 35?

It is difficult to be sure from your question, but you may have a slight misunderstanding of "Ct" value. Ct stands for cycle threshold¹. In a real time polymerase chain assay, a sample is ...
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