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Simpler than you think, its all about the high affinity of 2,3 BPG in binding to the deoxy-haemoglobin site. Now, when you deliver blood infusion into a closed circulatory system, you lower the 2,3BPG levels so that glycolysis in erythrocytes is shifted (by La Chatelie) to make more 2,3BPG. The total result is high 2,3PBG concentration(for a stable volume of ...

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