I know that blood contains white blood cells (which attack pathogens). Suppose we take half a liter of blood out of the body and implant the virus into it. Can the white blood cells in that half a liter of blood attack the virus and make antibodies?
The usual approach is to use a whole animal such as horses
The results of our research indicate that healthy horses immunized with the SARS-CoV F69 strain can be induced to generate effective, specific and neutralizing antibodies
and to prevent anaphylaxis
Heterogenous antisera used for treatment possibly result in anaphylactoid severe acute side-effects. To avoid the side-effects caused by horse antiserum, IgG against SARS-CoV was digested with pepsin and purified with anion-exchange separations to exclude the immunogenicity of Fc fragments and to retain the special activity of binding the antigen of F(ab')2 fraction. The titers of neutralizing F(ab')2 against SARS-CoV was detected at higher level (1:5120). And approximately 15 g F(ab')2 fragments were obtained from 1 litre antiserum, with the purity above 90%.